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L of total medium e.g 958 ml for ZYM 5052 50 x M 1.25 M Na 2 HPO 4 7H 2 O 335 g/L 1.25 M KH 2 PO 4 170 g/L 2.5 M NH 4 Cl 134 g/L 0.25 M Na 2 SO 4 35.5 g/L Add in sequence to 700 ml water and stir until all salts have been dissolved Occasionally crystals appear but they can be re dissolved in the microwave The pH of a 20 fold dilution in
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